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KMID : 0861020170320010025
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Korea Journal of Herbology
2017 Volume.32 No. 1 p.25 ~ p.31
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A study of the Mori Radicis Cortex pre-treatment on transient ischemic brain injury in mice
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Chung Byung-Woo
Lim Jae-Yu
Lee Se-Eun
Lee Byung-Ho
Lim Se-Hyun
Lim Chi-Yeon
Cho Su-In
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Abstract
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Objectives : Mori Radicis Cortex (MRC), the root epidermis of Morus alba L., has been traditionally used to treat lung-related diseases in Korean Medicine. The common of MRC is Mulberry bark Morus bark, and it`s pharmaceutical properties and taste are known as sweet and cold, and it promotes urination and reduce edema by reducing heat from the lungs and soothe asthma. In the present study, anti-apoptotic mechanism of MRC in middle cerebral artery occlusion (MCAO) model in mice.
Methods : Two-hundred grams of MRC was extracted with methanol at room temperature for 5 days, and this was repeated one time. After filtration, the methanol was removed using vacuum evaporator, then stored at -20℃ until use. C57BL/6 male mice were housed in an environment with controlled humidity, temperature, and light cycle. In order to determine beneficial effects of MRC on ischemia induced brain damage, infarct volume, neurological deficit scores, activities of several apoptosis-related proteins such as caspase-8, -9, Bcl-xL in MCAO-induced brains of mice were analyzed. Mice in MRC-treated groups were orally administered 30, 100, or 300 mg/kg of body weight for three consecutive days before commencing the MCAO procedure.
Results : Pre-treatment of MRC significantly reduced infarct volume in MCAO subjected mice applied with 300 ㎎/ ㎏ of MRC methanol extract, and MRC effectively inhibited Bcl-xL reduction and caspase-9 activation caused by MCAO-induced brain damage.
Conclusions : MRC showed neuro-protective effects by regulating apoptosis-related protein signals, and it can be a potential candidate for the therapy of ischemia-induced brain damage.
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KEYWORD
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Mori Radicis Cortex, middle cerebral artery occlusion, cerebral blood flow
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